Percharde Lab

Publications

[2025]

CRISPRa-mediated disentanglement of the Dux-MERVL axis in the 2C-like state, totipotency and cell death.
Chammas P, Xie SQ, Sepulveda-Rincon LP, Leeke BJ, Dore MH, Dormann D, Wagner RT, McManus MT, Karimi MM, Young G, Percharde M (2025)
SCIENCE ADVANCES
https://www.science.org/doi/10.1126/sciadv.adu9092

The exit of naïve pluripotency contains a lipid metabolism-induced checkpoint for genome integrity.
de Souza RA, Barneda D, Karimlou D, Bovee NGP, Zheng Y, Kahlman EJEM, Lopes Novo C, Ellis JK, Leeke BJ, Bangalore MP, Liu Z, Sousa BC, Lopez Clavijo AF, Jansen JH, Barahona M, Percharde M, Keun HC, Christian M, Marks H, Azuara V* (2025)
CELL REPORTS
https://doi.org/10.1016/j.celrep.2025.116654

A dual enhancer-attenuator element ensures transient Cdx2 expression during mouse posterior body formation.
Amblard I, Baranasic D, Xie SQ, Moyon B, Percharde M, Lenhard B, Metzis V (2025)
DEVELOPMENTAL CELL
https://www.cell.com/developmental-cell/fulltext/S1534-5807(25)00361-2

Emerging roles for the nucleolus in development and stem cells.
Leeke BJ, Staffhorst I, Percharde M (2025)
DEVELOPMENT
https://doi.org/10.1242/dev.204696

PREPRINT
Inhibition of cytosolic DNA sensing and transposon activity safeguards pluripotency.
Garcia-Llagostera F, Putman ALK, Choromidis A, Leeke BJ, Stanik K, Ramos-Guzmán A, Moyon B, Gil J, Barr AR, Percharde M (2025)
BIORXIV
https://www.biorxiv.org/content/10.1101/2025.03.26.645264v1

SMARCA4 regulates the NK-mediated killing of senescent cells.
Reen V, D’Ambrosio M, Søgaard PP, Tyson K, Leeke BJ, Clément I, Dye ICA, Pombo J, Kuba A, Lan Y, Burr J, Bomann IC, Kalyva M, Birch J, Khadayate S, Young G, Provencher D, Mes-Masson A-M, Vernia S, McGranahan N, Brady HJM, Rodier F, Nativio R, Percharde M, McNeish IA, Gil J (2025)
SCIENCE ADVANCES
https://www.science.org/doi/10.1126/sciadv.adn2811

[2024]

PREPRINT
CRISPRa-mediated disentanglement of the Dux-MERVL axis in the 2C-like state, totipotency and cell death.
Chammas P, Xie SQ, Sepulveda-Rincon LP, Leeke BJ, Dore MH, Dormann D, Wagner RT, McManus MT, Karimi MM, Young G, Percharde M (2024)
BIORXIV
https://www.biorxiv.org/content/10.1101/2024.11.25.625195v1

PREPRINT
The exit of naïve pluripotency contains a lipid metabolism-induced checkpoint for genome integrity.
de Souza RA, Barneda D, Karimlou D, Bovee NGP, Zheng Y, Kahlman EJEM, Lopes Novo C, Ellis JK, Leeke BJ, Bangalore MP, Liu Z, Sousa BC, Lopez Clavijo AF, Jansen JH, Barahona M, Percharde M, Keun HC, Christian M, Marks H, Azuara V* (2024)
BIORXIV
https://www.biorxiv.org/content/10.1101/2024.09.22.613751v1

[2022]

Nucleolar-based Dux repression is essential for embryonic 2-cell stage exit.
Xie SX, Leeke BJ, Whildling C, Wagner RT, Garcia-Llagostera F, Chammas P, Cheung NT-F, Dormann D, McManus MT, Percharde M* (2022)
GENES & DEVELOPMENT
https://doi.org/10.1101/gad.349172.121

[2021]

PREPRINT
Nucleolar-based Dux repression is essential for 2-cell stage exit.
Xie SX, Leeke BJ, Whildling C, Wagner RT, Garcia-Llagostera F, Chammas P, Cheung NT-F, Dormann D, McManus MT, Percharde M* (2021)
BIORXIV
https://www.biorxiv.org/content/10.1101/2021.11.11.468235v1

Depletion of nuclear LINE1 RNA in mouse ESCs and embryos.
Percharde M*, Lin CJ, Ramalho-Santos M (2021)
STAR PROTOCOLS
*Lead Contact
https://doi.org/10.1016/j.xpro.2021.100726

Homotypic clustering of L1 and B1/Alu repeats compartmentalizes the 3D genome.
Lu JY, Chang L, Li T, Wang T, Yin Y, Zhan G, Zhang K, Percharde M, Wang L, Peng Q, Yan P, Zhang H, Chang Z, Hou Y, Zhu B, Ramalho-Santos M, Li P, Xie W, Na J, Sun Y, Shen X (2021)
CELL RESEARCH
https://doi.org/10.1038/s41422-020-00466-6

[2020]

Delineating the early transcriptional specification of the mammalian trachea and esophagus.
Kuwahara A, Lewis AE, Coombes C, Leung FS, Percharde M, Bush JO (2020)
ELIFE
https://doi.org/10.7554/eLife.55526

Genomic Repeats Categorize Genes with Distinct Functions for Orchestrated Regulation.
Lu JY, Shao W, Chang L, Yin Y, Li T, Zhang H, Hong Y, Percharde M, Guo L, Wu Z, Liu L, Liu W, Yan P, Ramalho-Santos M, Sun Y, Shen X (2020)
CELL REPORTS
https://doi.org/10.1016/j.celrep.2020.02.048

What Doesn't Kill You Makes You Stronger: Transposons as Dual Players in Chromatin Regulation and Genomic Variation.
Percharde M*, Sultana S, & Ramalho-Santos M* (2020)
BIOESSAYS
*co-corresponding
https://doi.org/10.1002/bies.201900232

[2019]

PREPRINT
L1 and B1 repeats blueprint the spatial organization of chromatin.
Lu JY, Chang L, Li T, Wang T, Yin Y, Zhan G, Zhang K, Percharde M, Wang L, Peng Q, Yan P, Zhang H, Han X, Bi X, Shao W, Hong Y, Wu Z, Wang P, Li W, Zhang J, Chang Z, Hou Y, Li P, Ramalho-Santos M, Na J, Xie W, Sun Y, Shen X (2019)
BIORXIV
https://doi.org/10.1101/802173

Maternal Vitamin C regulates reprogramming of DNA methylation and germline development.
DiTroia SP, Percharde M, Guerquin MJ, Wall E, Collignon E, Ebata KT, Mesh K, Mahesula S, Agathocleous M, Laird DJ, Livera G, Ramalho-Santos M (2019)
NATURE
https://doi.org/10.1038/s41586-019-1536-1

[2018]

A LINE1-Nucleolin partnership regulates early development and ESC identity.
Percharde M, Lin CJ, Yin Y, Guan J, Peixoto GA, Bulut-Karslioglu A, Biechele S, Huang B, Shen X, Ramalho-Santos M (2018)
CELL
https://doi.org/10.1016/j.cell.2018.05.043

The transcriptionally permissive chromatin state of ES cells is acutely tuned to translational output.
Bulut-Karslioglu A, Macrae TA, Oses-Prieto JA, Covarrubias S, Percharde M, Ku G, Diaz A, McManus MT, Burlingame AL, Ramalho-Santos M (2018)
CELL STEM CELL
https://doi.org/10.1016/j.stem.2018.02.004

[2017]

Global hypertranscription in the mouse embryonic germline.
Percharde M, Wong P, Ramalho-Santos M (2017)
CELL REPORTS
https://doi.org/10.1016/j.celrep.2017.05.036

Hypertranscription in development, stem cells and regeneration.
Percharde M, Bulut-Karslioglu A, Ramalho-Santos M (2017)
DEVELOPMENTAL CELL
https://doi.org/10.1016/j.devcel.2016.11.010

[2016]

YAP induces human naïve pluripotency.
Qin H, Hejna M, Liu Y, Percharde M, Wossidlo M, Blouin L, Durruthy-Durruthy J, Wong P, Qi Z, Yu J, Qi LS, Sebastiano V, Song JS, Ramalho-Santos M (2016)
CELL REPORTS
https://dx.doi.org/10.1016/j.celrep.2016.02.036

[2009-2013]

Essential roles for the nuclear receptor coactivator Ncoa3 in pluripotency.
Percharde M, Azuara V (2013)
CELL CYCLE
https://dx.doi.org/10.4161/cc.23377

Ncoa3 functions as an essential Esrrb coactivator to sustain embryonic stem cell self-renewal and reprogramming.
Percharde M, Lavial F, Ng JH, Kumar V, Tomaz RA, Martin N, Yeo JC, Gil J, Prabhakar S, Ng HH, Parker MG, Azuara V (2012)
GENES & DEVELOPMENT
https://dx.doi.org/10.1101/gad.195545.112

The context and potential of epigenetics in oncology.
Lopez M, Percharde M, Coley HM, Webb A, Crook T (2009), 100: 571-578.
BRITISH JOURNAL OF CANCER
https://dx.doi.org/10.1038/sj.bjc.6604930