Percharde Lab

Publications

[2025]

CRISPRa-mediated disentanglement of the Dux-MERVL axis in the 2C-like state, totipotency and cell death. SCIENCE ADVANCES (in press)
Chammas P, Xie SQ, Sepulveda-Rincon LP, Leeke BJ, Dore MH, Dormann D, Wagner RT, McManus MT, Karimi MM, Young G, Percharde M (2025)
Originally posted on bioRxiv (2024).
https://www.biorxiv.org/content/10.1101/2024.11.25.625195v1

The exit of naïve pluripotency contains a lipid metabolism-induced checkpoint for genome integrity. CELL REPORTS (in press)
de Souza RA, Barneda D, Karimlou D, Bovee NGP, Zheng Y, Kahlman EJEM, Lopes Novo C, Ellis JK, Leeke BJ, Bangalore MP, Liu Z, Sousa BC, Lopez Clavijo AF, Jansen JH, Barahona M, Percharde M, Keun HC, Christian M, Marks H, Azuara V* (2025)
Originally posted on bioRxiv (2024).
https://www.biorxiv.org/content/10.1101/2024.09.22.613751v1

A dual enhancer-attenuator element ensures transient Cdx2 expression during mouse posterior body formation. DEVELOPMENTAL CELL
Amblard I, Baranasic D, Xie SQ, Moyon B, Percharde M, Lenhard B, Metzis V (2025)
https://www.cell.com/developmental-cell/fulltext/S1534-5807(25)00361-2

Emerging roles for the nucleolus in development and stem cells. DEVELOPMENT
Leeke BJ, Staffhorst I, Percharde M (2025)
https://doi.org/10.1242/dev.204696

Inhibition of cytosolic DNA sensing and transposon activity safeguards pluripotency. BIORXIV
Garcia-Llagostera F, Putman ALK, Choromidis A, Leeke BJ, Stanik K, Ramos-Guzmán A, Moyon B, Gil J, Barr AR, Percharde M (2025)
https://www.biorxiv.org/content/10.1101/2025.03.26.645264v1

SMARCA4 regulates the NK-mediated killing of senescent cells. SCIENCE ADVANCES
Reen V, D’Ambrosio M, Søgaard PP, Tyson K, Leeke BJ, Clément I, Dye ICA, Pombo J, Kuba A, Lan Y, Burr J, Bomann IC, Kalyva M, Birch J, Khadayate S, Young G, Provencher D, Mes-Masson A-M, Vernia S, McGranahan N, Brady HJM, Rodier F, Nativio R, Percharde M, McNeish IA, Gil J (2025)
https://www.science.org/doi/10.1126/sciadv.adn2811

[2024]

CRISPRa-mediated disentanglement of the Dux-MERVL axis in the 2C-like state, totipotency and cell death (preprint). BIORXIV
Chammas P, Xie SQ, Sepulveda-Rincon LP, Leeke BJ, Dore MH, Dormann D, Wagner RT, McManus MT, Karimi MM, Young G, Percharde M (2024)
https://www.biorxiv.org/content/10.1101/2024.11.25.625195v1

The exit of naïve pluripotency contains a lipid metabolism-induced checkpoint for genome integrity (preprint. BIORXIV
de Souza RA, Barneda D, Karimlou D, Bovee NGP, Zheng Y, Kahlman EJEM, Lopes Novo C, Ellis JK, Leeke BJ, Bangalore MP, Liu Z, Sousa BC, Lopez Clavijo AF, Jansen JH, Barahona M, Percharde M, Keun HC, Christian M, Marks H, Azuara V* (2024)
https://www.biorxiv.org/content/10.1101/2024.09.22.613751v1

[2022]

Nucleolar-based Dux repression is essential for embryonic 2-cell stage exit. GENES & DEVELOPMENT
Xie SX, Leeke BJ, Whildling C, Wagner RT, Garcia-Llagostera F, Chammas P, Cheung NT-F, Dormann D, McManus MT, Percharde M* (2022)
https://doi.org/10.1101/gad.349172.121

[2021]

Nucleolar-based Dux repression is essential for 2-cell stage exit (preprint). BIORXIV
Xie SX, Leeke BJ, Whildling C, Wagner RT, Garcia-Llagostera F, Chammas P, Cheung NT-F, Dormann D, McManus MT, Percharde M* (2021)
https://www.biorxiv.org/content/10.1101/2021.11.11.468235v1


Depletion of nuclear LINE1 RNA in mouse ESCs and embryos. STAR PROTOCOLS
Percharde M*, Lin CJ, Ramalho-Santos M (2021)
*Lead Contact
https://doi.org/10.1016/j.xpro.2021.100726


Homotypic clustering of L1 and B1/Alu repeats compartmentalizes the 3D genome. CELL RESEARCH
Lu JY, Chang L, Li T, Wang T, Yin Y, Zhan G, Zhang K, Percharde M, Wang L, Peng Q, Yan P, Zhang H, Chang Z, Hou Y, Zhu B, Ramalho-Santos M, Li P, Xie W, Na J, Sun Y, Shen X (2021)
https://doi.org/10.1038/s41422-020-00466-6
Preprint here

[2020]

Delineating the early transcriptional specification of the mammalian trachea and esophagus. ELIFE
Kuwahara A, Lewis AE, Coombes C, Leung FS, Percharde M, Bush JO (2020)
https://doi.org/10.7554/eLife.55526


Genomic Repeats Categorize Genes with Distinct Functions for Orchestrated Regulation. CELL REPORTS
Lu JY, Shao W, Chang L, Yin Y, Li T, Zhang H, Hong Y, Percharde M, Guo L, Wu Z, Liu L, Liu W, Yan P, Ramalho-Santos M, Sun Y, Shen X (2020)
https://doi.org/10.1016/j.celrep.2020.02.048


What Doesn't Kill You Makes You Stronger: Transposons as Dual Players in Chromatin Regulation and Genomic Variation. BIOESSAYS
Percharde M*, Sultana S, & Ramalho-Santos M* (2020)
*co-corresponding
https://doi.org/10.1002/bies.201900232

[2019]

L1 and B1 repeats blueprint the spatial organization of chromatin (preprint). BIORXIV
Lu JY, Chang L, Li T, Wang T, Yin Y, Zhan G, Zhang K, Percharde M, Wang L, Peng Q, Yan P, Zhang H, Han X, Bi X, Shao W, Hong Y, Wu Z, Wang P, Li W, Zhang J, Chang Z, Hou Y, Li P, Ramalho-Santos M, Na J, Xie W, Sun Y, Shen X
https://doi.org/10.1101/802173


Maternal Vitamin C regulates reprogramming of DNA methylation and germline development. NATURE
DiTroia SP, Percharde M, Guerquin MJ, Wall E, Collignon E, Ebata KT, Mesh K, Mahesula S, Agathocleous M, Laird DJ, Livera G, Ramalho-Santos M (2019)
https://doi.org/10.1038/s41586-019-1536-1

[2018]

A LINE1-Nucleolin partnership regulates early development and ESC identity. CELL
Percharde M, Lin CJ, Yin Y, Guan J, Peixoto GA, Bulut-Karslioglu A, Biechele S, Huang B, Shen X, Ramalho-Santos M (2018)
https://doi.org/10.1016/j.cell.2018.05.043


The transcriptionally permissive chromatin state of ES cells is acutely tuned to translational output. CELL STEM CELL
Bulut-Karslioglu A, Macrae TA, Oses-Prieto JA, Covarrubias S, Percharde M, Ku G, Diaz A, McManus MT, Burlingame AL, Ramalho-Santos M (2018)
https://doi.org/10.1016/j.stem.2018.02.004

[2017]

Global hypertranscription in the mouse embryonic germline. CELL REPORTS
Percharde M, Wong P, Ramalho-Santos M (2017)
https://doi.org/10.1016/j.celrep.2017.05.036


Hypertranscription in development, stem cells and regeneration. DEVELOPMENTAL CELL
Percharde M, Bulut-Karslioglu A, Ramalho-Santos M (2017)
https://doi.org/10.1016/j.devcel.2016.11.010

[2016]

YAP induces human naïve pluripotency. CELL REPORTS
Qin H, Hejna M, Liu Y, Percharde M, Wossidlo M, Blouin L, Durruthy-Durruthy J, Wong P, Qi Z, Yu J, Qi LS, Sebastiano V, Song JS, Ramalho-Santos M (2016)
https://dx.doi.org/10.1016/j.celrep.2016.02.036

[2009-2013]

Essential roles for the nuclear receptor coactivator Ncoa3 in pluripotency. CELL CYCLE
Percharde M, Azuara V (2013)
https://dx.doi.org/10.4161/cc.23377


Ncoa3 functions as an essential Esrrb coactivator to sustain embryonic stem cell self-renewal and reprogramming. GENES & DEVELOPMENT
Percharde M, Lavial F, Ng JH, Kumar V, Tomaz RA, Martin N, Yeo JC, Gil J, Prabhakar S, Ng HH, Parker MG, Azuara V (2012)
https://dx.doi.org/10.1101/gad.195545.112


The context and potential of epigenetics in oncology. BRITISH JOURNAL OF CANCER
Lopez M, Percharde M, Coley HM, Webb A, Crook T (2009), 100: 571-578
https://dx.doi.org/10.1038/sj.bjc.6604930